CANDIDATURES
Recherche un stage en Développement Informatique du 7 Avril au 25 Juillet – lien
Recherche d’alternance – BTS Bioanalyses en laboratoire de contrôle- lien
Candidature de stage technicien de laboratoire en contrôle qualité- du 12 au 27 juin 2025 – lien
Etudiante en L2 Sciences de la Vie avec spécialisation en biologie cellulaire et physiologie à l’UT3, je suis à la recherche d’un stage en institut de biotechnologies. période allant du 19 mai au 15 juin 2025. – lien
En troisième année de BUT Mesures Physiques, en spécialité Matériaux et Contrôles Physico-Chimiques. Récemment admise & l’école d’ingénieur PAGORA INP de Grenoble, se spécialisant dans le papier, ainsi que dans les biomatériaux. De ce fait, je suis activement à la recherche d’un contrat d’alternance. – lien
OFFRES
Offres de thèse au sein des laboratoires du CEA
Le service de formation par la recherche et de l’emploi scientifique de l’Institut national des sciences et techniques nucléaires – INSTN, administré par le CEA, nous informe que plus de 400 sujets de thèses sont proposés à l’étude pour à la rentrée 2025. Les informations concernant ces offres de poursuite d’études en thèse sont accessibles sur ce lien .
Pour obtenir plus d’information, vous pouvez contacter Elena Ceccarelli par téléphone au 01 69 08 87 04 ou par mail à elena.ceccarelli@cea.fr.
PhD Thesis Proposal − GEPEA – Integrated Modeling and Process Optimization of Biocalcification in Gephyrocapsa huxleyi : Toward Sustainable CO2 Storage Solutions – lien
IFPEN – Towards a comprehensive understanding of reaction mechanisms of « Lytic polysaccharide monooxygénases » (LPMO) for an optimized hydrolysis of recalcitrant lignocellulose substrates
The production of bioethanol from lignocellulosic substrates depends on efficient enzymatic hydrolysis, which is one of the most expensive steps in the process. The Trichoderma reesei enzymatic cocktail is one of the most efficient, but certain substrates, such as softwood or Miscanthus, remain difficult to degrade with a low sugar yield. IFPEN has been working for many years on improving the cellulase cocktail.
It has been shown that Lytic Polysaccharide Mono Oxygenases (LPMO) are capable of boosting cellulase activity. These copper metalloenzymes which oxidize sugar polymers can constitute a way to improve the cocktail of cellulases on recalcitrant substrates such as sotfwoods but their particularities complicate their industrial implementation. Many parameters can indeed impact their activity and capability to enhance cellulase activity, such as the nature of the co-substrate and electron donor, regioselectivity, pH or the type of substrate.
The thesis aims to identify important features of the primary structure of LPMO which influence their reactivities towards cellulose, the necessary reduction of the copper ion, and their interaction with the substrate. To this end, two wild type enzymes and several variants supposed to impact substrate affinity and reactivity will be generated and biochemically characterized. In addition, the capacity of these LPMOs to act in synergy with T. reesei cellulases on a real substrate will be evaluated.
As the present PhD thesis is part of a collaborative project, the enzymes will moreover be characterized by partner laboratories with expertise in biophysical methods, such as NMR, electrochemistry and EPR (electron paramagnetic resonance) as well as in theoretical modelling. This multidisciplinary approach, combining fundamental and applied aspects, will thus allow gathering complementary data to acquire essential knowledge on LPMO which is key for improvement of LPMO for industrial processes.
Keywords : cellulose hydrolysis, biofuel, cellulases, LPMO, enzymology, metalloenzyme, Pichia pastoris, Trichoderma reesei
Competences required :
Master’s degree in biology or biochemistry. The candidate should have very good theoretical and practical knowledge of biochemical methods and enzymology. Basic knowledge of structural biology and biophysical methods are desirable. Fluency in English, basic knowledge of French
Soft skills : Team work, communication skills, curiosity
Please send applications to : senta.blanquet@ifpen.fr OR simon.arragain@ifpen.fr
