Lundi, 4 décembre⋅14:00
Soutenance de thèse Thibault Malfoy
La thèse s’intitule : “La promiscuité de protéines : facteur clef pour la (ré-)assimilation et la valorisation de la molécule plateforme non naturelle 2,4-dihydroxybutyrate chez Escherichia coli“.
Jeudi, 7 décembre⋅13:00
Séminaire Prof. Artur Gora – salle 401
Artur Góra Head of the Tunneling Group at Biotechnology Centre Silesian University of Technology, Gliwice, Poland
Title: Water molecules as a key for enzymes interior understanding and reshaping abstract: Water molecules maintain enzymes’ structures, functions, stabilities and dynamics. Therefore, they are ideal probes for investigation of enzymes’ properties which allows us to understand the mechanisms responsible for macromolecules interactions, signal transduction and catalysis. The analysis of water molecules’ distribution and trajectories allows to study proteins’ interactions with small molecules, composition, tunnel and cavities dynamics and functionality, transportation network and location of key residues. Such studies of the dynamics of tunnels, cavities and other types of intramolecular voids buried inside macromolecule’s core remain a challenging task due to the ephemeral character of these elements. Moreover, the experimental methods hardly provide insight into accessibility of such structures, transport of small ligand(s) or solvent exchange between protein interior and the environment. We would like to present a comprehensive overview of several studies performed by us in the last few years using water tracking software which provided insight and understanding into proteins’ function and evolution which then facilitated enzyme redesign and drug discovery. By combining molecular dynamic simulations with our software, we got access to information about flow direction, and regions in enzymes in which small molecules are stuck or trapped. By analysing the local distribution analysis, we were able to determine the approximate energy profile of a particular passage and finally understand changes in enzyme performance due to particular mutations. The extensive comparison of interior dynamics within the enzyme family helps us to understand tunnels network evolution. The analysis of the protein interior from the perspective of water molecules can be used as a versatile and exhaustive approach that enables enzymes understanding and re-engineering.
Jeudi, 7 décembre⋅13:30 – Amphi Riquet
Cérémonie de remerciements igem
Mercredi, 13 décembre⋅08:30 à 10:30
Petit déjeuner de Noël 🎄
Jeudi, 14 décembre⋅09:30 à 12:00 – salle 401
Animation GT Hétérogénéité
(présentation 20 min + 20 min d’échanges, salle 401
9h30-10h15 : Delphine Lestrade: “Cytométrie spectrale : présentation de la technologie, comparaison avec la cytométrie conventionnelle et principe de l’analyse spectrale”
10h15-11h : Jean-Luc Parrou : “Importance du partitionnement pour révéler et quantifier les minorités (invisibles)”
11h-11h45 : Floriant Belvert : “Single cell metabolomics : state of the art and prespective for the study of metabolic heterogeneity”
11h45-12h : Discussion générale
animateurs du GT et contact Didier Zerbib & Jean Pascal Capp
Lundi, 18 décembre⋅14:00 – école de Purpan
Mon projet est fruit d’un partenariat entre les équipes toulousaines BLADE (Toulouse Biotechnology Institute-INSA Toulouse), l’équipe NGN (ToxAlim-INRAE) et le groupe industriel lillois Lesaffre International. Il s’intitule : « Exploration du potentiel santé des souches de Lactococcus lactis productrices de GABA : vers de nouvelles stratégies thérapeutiques dans la gestion de l’hypersensibilité viscérale », présenté en langue française
Mercredi, 20 décembre⋅09:00 – salle 401
Soutenance de thèse de Romain Launay
Computational characterization and understanding of protein assemblies: the case of the Escherichia coli Ubi metabolon involved in ubiquinone biosynthesis” and will be defended in French
Fermeture INSA : Du vendredi 22 décembre 2023 au soir au mardi 2 janvier 2024 inclus
